animals gfap cre mice expressing cre  (Jackson Laboratory)

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Depletion of BIS in astrocytes aggravates reactive gliosis following photothrombotic brain injury

doi: 10.4196/kjpp.25.361

Figure Lengend Snippet: (A) Schematic illustration of the generation of BIS-aKO mice. BIS f/f mice were crossed with GFAP-Cre mice to generate BIS f/+ ;GFAP-Cre mice. These mice were then crossed with BIS f/f mice to obtain BIS f/f ;GFAP-Cre mice. Subsequently, BIS f/f mice were bred with BIS f/f ;GFAP-Cre mice to generate littermate control mice (BIS f/f ) and astrocyte-specific BIS knockout mice (BIS f/f ;GFAP-Cre, hereafter referred to as BIS-aKO). (B) The expression levels of Bis mRNA in various brain cell types from the P7 mouse cerebral cortex analyzed using dataset GSE52564 (n = 2 per cell type). (C–J) Representative images of BIS and GFAP detected by DAB staining in resting astrocytes of control and BIS-aKO mouse brains. In control mice, BIS (C, D) and GFAP (G, H) were expressed in astrocytes of white matter tracts as well as in astrocytes surrounding blood vessels in the corpus callosum. In BIS-aKO mice, BIS expression (E, F) was abolished in astrocytes of both white matter tracts and perivascular regions, whereas GFAP expression (I, J) was preserved. Higher-magnification views of the boxed areas are shown in D, F, H, J. Arrows indicate astrocytes in white matter tracts; arrowheads indicate perivascular astrocytes. (K–P) Representative immunofluorescence images of BIS and GFAP in resting astrocytes of the corpus callosum in control and BIS-aKO mouse brains. The images show the loss of BIS expression and the preservation of GFAP expression in astrocytes of BIS-aKO mice (N–P) compared with controls (K–M). Arrows indicate astrocytes in white matter tracts; arrowheads indicate perivascular astrocytes. Scale bars = 200 µm for C, E, G, I; 50 μm for D, F, H, J; 50 μm for K–P. Data are presented as the mean ± standard error of the mean (SEM). BIS, Bcl-2-interacting cell death suppressor; OPC, oligodendrocyte precursor cells; NFO, newly formed oligodendrocytes; MO, myelinating oligodendrocytes; LV, lateral ventricle.

Article Snippet: Mice homozygous for exon 4-floxed Bis (BIS f/f ) were crossed with GFAP-Cre transgenic mice (B6.Cg-Tg(Gfap-cre)73.12Mvs/J, The Jackson Laboratory) to generate BIS f/+ ;GFAP-Cre offspring, which were subsequently bred with BIS f/f mice to obtain BIS f/f ; GFAP-Cre mice.

Techniques: Control, Knock-Out, Expressing, Staining, Immunofluorescence, Preserving

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: GFAP + FOXF2 + ependymal cells promote blood–brain barrier repair via DLL4–NOTCH signaling after neural injury

doi: 10.1073/pnas.2520352123

Figure Lengend Snippet: Single-cell transcriptomic profiling of the SVZ region following stroke. ( A ) Experimental workflow for single-cell RNA analysis in the SVZ. The SVZ samples used for single-cell sequencing were obtained from three different mice in each group. ( B ) UMAP plot illustrating the distribution of individual cell subsets. ( C ) Violin plot displaying cell type–specific marker expression in the clusters. ( D ) The proportional abundance of each cell subgroup. ( E ) Sankey diagram illustrating the differences in both cell numbers and cell proportions between the sham and 7 dpi groups for each cell type. ( F ) The ependymal cell subset was further subdivided to characterize the cell subpopulations. ( G ) Violin plot of Foxj1 and Gfap expression, with Gfap categorized into low expression (clusters 1 and 2), high expression (cluster 3), and medium expression in other clusters. ( H ) Enrichment analysis of the biological processes associated with the high- and low-expression Gfap subpopulations. ( I ) Comparison of the proportions of ependymal cell subpopulations between the sham group and the 7 dpi group. ( J ) BP analysis of three ependymal cell subpopulations (clusters 5, 6, and 7) whose proportions increased at 7 dpi. ( K ) GSEA enrichment analysis identifying core genes that regulate angiogenesis pathways in cluster 7 (C7). ( L ) Violin plot validating the expression specificity of key angiogenesis molecules in ependymal cell subpopulations. ( M ) The scatter plot confirms that Foxf2 is highly expressed only in the ependymal cell subpopulation C7.

Article Snippet: Foxf2 fl/fl mice, Dll4 fl/fl mice, and Gfap -Cre mice originated from Cyagen Company (Suzhou, China).

Techniques: Single Cell, Sequencing, Marker, Expressing, Comparison